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KMID : 0616120090400040351
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Korean Journal of Pharmacognosy
2009 Volume.40 No. 4 p.351 ~ p.356
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Effects of Berberine on 6-hydroxydopamine-induced Parkinsonism in Rats
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Kwon Ik-Hyun
Choi Hyun-Sook
Shin Kun-Seong
Hwang Bang-Yeon
Lee Myung-Koo
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Abstract
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Many isoquinoline alkaloids including berberine lower dopamine content by reducing tyrosine hydroxylase (TH)
activity and aggravate L-DOPA-induced cytotoxicity in PC12 cells. In this study, the effects of berberine on 6-hydroxydopamine (6-OHDA)-induced cytotoxicity in PC12 cells and on unilateral 6-OHDA-lesioned rat models were investigated. Berberine at 10-30 μM did not affect cell viability in PC12 cells. However, berberine at concentrations higher than 50μM caused cytotoxicity at 24 h. Berberine (10-50 μM) also enhanced 6-OHDA (10-50 μM)-induced cytotoxicity at 24 h compared to 6-OHDA alone with an apoptotic process. In addition, treatment with berberine (5 and 30 mg/kg, i.p.) for three weeks showed a dopaminergic cell loss in substantia nigra of 6-OHDA-lesioned rats: 30 mg/kg berberine was more intensive cytotoxic. The levels of dopamine were also decreased by berberine (5 and 30 mg/kg) in the ipsilateral substantia nigra of 6-OHDA-lesioned rats. These results suggest that berberine aggravated 6-OHDA-induced cytotoxicity in PC12 cells and treatment with berberine (5 and 30mg/kg) in 6-OHDA-lesioned rats also enhanced the degeneration of dopaminergic cell death and the decrease in dopamine levels in substantia nigra. Therefore, the long-term L-DOPA therapeutic patients with isoquinoline compounds including berberine may need to be checked for the adverse symptoms.
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KEYWORD
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Berberine, 6-Hydroxydopamine-induced cytotoxicity, Dopamine, PC12 cells, 6-Hydroxydopamine-Lesioned rats
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